ISO 



with a thin layer of the dextrin solution. The paraffin 

 sections are then arranged in rows on the layer of dextrin, 

 and pressed against the plate with the finger. The plate 

 with the sections fixed to it is now placed in the warm oven, 

 the temperature of which should not exceed 40 C., where 

 it remains from 4 to 24 hours. The plate is then taken 

 from the warm oven, warmed over a flame until the paraf- 

 fin about the sections begins to melt, and transferred to a 

 tray containing xylol or toluol, where it remains until the 

 paraffin is dissolved. It is then transferred to a tray 

 containing 95% alcohol. In the course of a few minutes 

 the plate is taken out, and the alcohol drained off. The 

 sections are now covered with a thin layer of solution No. 

 2, after which the plate is set aside until the photoxolyn or 

 celloidin dries. The plate can now be placed in the stain 

 (Boehmer's hsematoxylin and eosin for instance). After 

 staining, washing, and dehydrating, the sections are cleared 

 in oil of origanum. It should be stated that in the 

 stains or in the water used for washing off the stains, the 

 thin layer of photoxolyn or celloidin, to which the sections 

 adhere, separates from the glass plate ; the film may then 

 be taken up from two sides, and transferred from one solu- 

 tion to another, until cleared in the oil of origanum. 

 With a small pair of scissors the sections can now be cut 

 out> and mounted in balsam. 



