152 



I 



Embedded in 



4 



v v 



Celloidin. Paraffin. 



Distilled water. Fix to slide or cover with 



albumen fixative or dextrin solution. 



. I 



Warm until paraffin melts. 



V 

 Dissolve paraffin in toluol or xylol. 



Wash in absolute alcohol. 



Wash in 95% alcohol. 



V 

 Wash in distilled water. 



; , * 



Boehmer's heematoxylin solution for 5 to 30 minutes. 



I- 



0.5% potash alum solution 5 minutes. This develops stain, 

 and acts as a mortant. 



Wash in distilled water. 



Dehydrate in alcohol. 



V 

 Clear in oil of bergamot. 



V V 



Celloidin sections. Sections fixed to slide or cover. 



I I 



Mount in balsam. Pass through xylol, and mount in balsam. 



Ehrlich's Haematoxylin Solution. 



This solution can be kept for a long time, it seems to 

 improve with age. 



