153 



Hsematoxylin crystals, . . . . 2 grms. 



Absolute alcohol, . . . . . . 20 c. c. 



Glycerine, . 100 c. c. 



Distilled water, ...... 100 c. c. 



Absolute alcohol, . . . . . . 80 c. c. 



Glacial acetic acid, . . . . . . 10 c. c. 



Potash alum to saturation. 



Mix the distilled water, the glycerine, and 80 c. c. of 

 the absolute alcohol, and the 10 c. c. of glacial acetic acid. 

 Dissolve the haematoxylin crystals in 20 c. c. of absolute 

 alcohol, add to the above solution, and shake well for 

 several minutes. 



The solution so obtained should have a reddish color, 

 and is now to be saturated with the alum ; filter at the end 

 of 24 hours. 



Ehrlich's hsematoxylin needs to mature from 1 to 2 

 months before it can be used. Steps for staining are as 

 follows : 



1. Sections remain in the stain from 10 to 30 minutes 

 (they do not easily overs tain). 



2. Wash in distilled water, dehydrate, clear in oil of 

 cloves or bergarnot, and mount in balsam. 



Delafield's Haematoxylin Solution. 



The formula is taken from Behren's Tabellen. 



Hsematoxylin crystals, ..... 4 grms. 



Absolute alcohol, 25 c. c. 



Ammonium alum, ...... 52 grms. 



Distilled water, . . . . . . 400 c. c. 



Glycerine, 100 c. c. 



Methyl alcohol, ...... 100 c. c. 



The hsematoxylin crystals are dissolved in the abso- 

 lute-alcohol, the alum in the hot water; as soon as the 

 alum solution cools, add the haematoxylin solution. Allow 

 to stand in a wide vessel from 3 to 4 days, filter, and add 

 the glycerine and methyl alcohol. 



This hsematoxylin solution is generally used for stain- 

 ing tissues en masse. Before using, dilute the stain 5 to 10 

 times with distilled water. The tissues, which may be 

 hardened in Miiller's fluid, mercuric chloride, picric acid, 

 alcohol, or Flemming's or Hermann's solution, remain in the 



