157 



I 

 Rinse thoroughly in two tap waters and one distilled water. 



I 



Stain for 24 hours in - - 



Hsematoxylin crystals . . 1 grra. 



Absolute alcohol . . . 10 c. c. 



Distilled water ... 90 c. c. 



V 



AY ash sections in tap water for 10 to 15 min. 



V 



Place in 0.25% aqueous solution of permanganate of pot- 



ash until the gray matter begins to stand out 



(1 to 15 minutes). 



4 



Wash in distilled water. 



V 



Differentiate in the following solution - 



Hydric sulphite . . . about 5 to 10 c. c. 



Distilled water .... 100 c. c. 



until the gray matter has a light yellow color. 



I 



Wash in tap water for 1 to 2 hours. 



v 



Rinse sections in distilled water. 



Double stain in alum carmine for 1 to 1^2 hours, 

 wash in distilled water. 



Dehydrate. 



4 



Clear in Weigert's clearing fluid. 



4 



Mount in balsam. 



If sections have been kept for some time in alcohol, it 

 is advisable to place them for 24 hours in Miiller's fluid, 

 from which they are rinsed in distilled water, and stained 

 after any one of the above three methods. 



