160 



Flemming's Safranin Solution. 



Safranin . . . . 1 grm. 



Absolute alcohol . . . 10 c. c. 



Aniline water . . . . 90 c. c. 



The aniline water is prepared by shaking 5 to 8 c. c. 

 of aniline oil with 100 c. c. of distilled water, and filtering 

 through a wet filter paper. Dissolve the safranin in aniline 

 water, and add the alcohol. Filter before using. 



The steps for staining are as follows : 



Harden tissues in Flemming's or Hermann's solution. 



4 



Embed in paraffin. 



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Cut very thin sections, and fix to cover glasses. 



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Remove paraffin with toluol or xylol, and, after rinsing 

 sections in alcohol, transfer into distilled water. 



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Stain in Flemming's safranin solution for 24 to 48 hours. 



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Rinse in distilled water. 

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v 



Wash thoroughly in 95% alcohol (200 c. c.) to 

 which 1 or 2 drops of HC1 have been added. 



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Wash in alcohol, clear in oil of bergamot, pass through 

 xylol, a'nd mount in balsam. 



In tissues hardened and stained in the above way, the 

 chromatic network of the nucleus is well stained ; this 

 method is largely used for staining cells in process of 

 division. 



Gentian Violet Solution (Ehrlich and Bizzozero), Taken 

 from Lee's Vade Mecum. 



Gentian violet . . . . .' 1 part. 



Alcohol 15 parts. 



Aniline oil 3 parts. 



Distilled water .... 80 parts. 



