Mix the aniline oil with water, and filter through wet 

 filter paper. Dissolve gentian violet in aniline water, and 

 add the alcohol. 



The steps of the method are as follows : 



The tissues are hardened in Flemming's or Hermann's 

 solution. 



4 



Embed in paraffin, section, and fix sections to cover glasses ; 



remove paraffin from sections, and bring them 



into distilled water. 



4 



Stain in the above solution for 10 to 15 hours. 



Rinse in alcohol. 



V 



Wash in 1% aqueous sol. of chromic acid 30 seconds. 



4 



Wash in alcohol for a few seconds. 



I 



Again bring into 1% aqueous sol. of chromic acid 30 seconds. 



Wash in alcohol for 30 to 40 seconds. 



;-4 



Clear in oil of cloves; the oil needs to be changed once or 



twice, as much of the stain is given off in it. 



Sections remain in the oil until no more 



stain is given off. 



I 

 V 



Pass through xylol, and mount in balsam. 



Tissues hardened in alcohol need to be stained only 10 

 to 15 minutes ; the after treatment is the same as above 

 given. 



This method brings out clearly nuclear structure and 

 karyokinetic figures. 



Methylene blue, methyl violet, methyl green, and Bis- 

 mark Brown, may be made up as \% solutions in about 

 % alcohol. The tissues hardened in alcohol and mercuric 



chloride will be stained in 10 to 15 minutes ; tissues hard- 

 11 



