162 



ened in Flemming's or Hermann's solution in as many 

 hours. 



Wash sections thoroughly in alcohol, and mount in 

 balsam. 



As the acid aniline dyes are generally used for counter- 

 staining, they will be described under the head of double 

 and triple stains. 



DOUBLE STAINING. 



When certain colors are combined in a solution, or 

 used one after the other in staining, it has been found that 

 some elements of the tissues to be stained are colored by 

 one of the dyes used, while others show greater affinity for 

 other dyes. This fact is made use of in combining dyes 

 for double and triple staining. 



Haematoxylin and Eosin or Acid Fuchsin. 



The eosin solution used for double staining is made 

 after the following formula : 



Eosin . V.V . . . , . ' 1 grm. 

 Alcohol (60%) .. . , . . 100 c.c. 

 The acid fuchsin solution 

 Acid fuchsin . . ." . . 1 to 2 grms. 

 Distilled water . . 100 c.c. 



The steps for staining are as fallows : 



