Orange G and Haematoxylin (Rawitz). 



The tissues should be hardened in absolute alcohol, mercuric 

 chloride, or picric acid. 



4 



Embed in paraffin, and fix sections to cover glass. 



V 



Remove paraffin, and bring sections into distilled water. 

 I 



V 



Stain in a saturated aqueous solution of Orange G (filtered ; 

 for about 24 hours. 



I 



Rinse in distilled water. 



j 



Stain in Boehmer's haematoxylin solution for about 5 

 minutes. 



I 



Wash in distilled water. 



I 



Dehydrate in alcohol. 



I 



Clear in oil of bergamot. 



V 



Pass through xylol, and mount in balsam. 



This is certainly one of the best of double stains, 

 especially for glandular tissues. The nuclei are stained 

 with the hsematoxylin, the protoplasm with orange G. 



Safranin and Lichtgruen (Benda). 



Tissues hardened inFlemming's or Hermann's solution 

 are embedded in paraffin, and sections fixed to the cover 

 glass. Remove paraffin, and bring sections into distilled 

 water. 



Stain for about 24* hours in the following safranin 

 solution : 



Safranin 1 grm. 



Aniline water . . . 90 c. c. 



Absolute alcohol . 10 c. c. 



