166 



4. The sections are now transferred to distilled water, 

 in which the sections agnin assume a blue color, of a much 

 lighter hue, however. In the distilled water the sections 

 remain about 10 minutes. 



5. Counter-stain in a saturated aqueous solution of 

 safranin for about 30 minutes. 



6. Rinse in distilled water. 



7. Wash, and dehydrate quickly in 95% and absolute 

 alcohol. 



8. Clear in oil of bergamot, pass sections through 

 xylol, and mount in balsam. 



The axis cylinders are all stained blue, the myelin a 

 yellowish-red or orange color, and all nuclei red. 



Carmine and Aniline Blue (Duval). 



Tissues are stained in borax carmine in the usual way, 

 {ire then embedded in paraffin, sectioned, and sections fixed 

 to cover glasses. Remove paraffin from sections, and bring 

 them into absolute alcohol. 



Stain for 10 to 20 minutes in 



Aniline blue* saturated alcohol solution 10 drops. 



Absolute alcohol . . . . 10 c. c. 



Wash, and clear sections in turpentine, puss through 

 xylol, and mount in balsam. 



The central nervous system is well stained after this 

 method; the tissues may be hardened in bichloride of 

 mercury. The connective tissue elements are stained blue, 

 the nerve cells and axis cylinders a reddish-violet. 



Borax Carmine and Indigo Carmine fNorris and Shakes- 

 peare, taken from Bobm and Oppel's Tashenbuch 

 der inikroskopischeft Technic). 



Sol. A. Grind in a mortar. 



Carmine . . . . . . 2 grms. 



Borax . . . . . . 8 grms. 



Distilled water . . . . 130 c. c. 



Allow to stand for 24 hours, then filtor. 



Sol. B. Rub up in mortar. 



Indigo carmine ..... 8 grms. 



Borax ' 8 grms. 



Distilled water . . . . 130 c. c. 



Allow to stand for 24 hours, then filter. 



