-i6 7 - 



Before using, mix equal parts of sol. A and B. Stain 

 tissues for 20 minutes, wash in a concentrated aqueous sol. 

 of oxalic acid, rinse in absolute alcohol, dehyrate, and 

 mount in balsam. 



Tissues may be hardened in Miiller's fluid, thoroughly 

 washed in water, and embedded in celloidin. 



TR IPLE STAINING. 



The formula here given was first recommended by 

 Ehrlich, and used by him for staining blood preparations. 

 Biondi and Heidenhain have slightly modified it, to make 

 it more suitable for section staining. 



Acid fuchsin (sat. aqueous sol.) . 20 parts. 

 Methyl green (sat. aqueous sol.) . 50 parts. 

 Orange G (sat. aqueous sol.) . 100 parts. 



The acid fuchsin and orange Gr are mixed, the methyl 

 green is slowly added while constantly stirring. The 

 stain should not be filtered, and needs to be diluted with 

 distilled water 40 to 60 times before using. 



Steps for staining are as follows : 



Harden tissues in absolute alcohol or bichloride of mercury. 



I 



Embed in paraffin. 



I 



Fix sections to cover glasses. 



\, 



Remove paraffin, and bring sections into distilled water. 



v 



Stain for 4 to 24 hours. 



I 



Rinse in distilled water. 



Wash thoroughly in alcohol. 



V 

 Dehydrate, and clear in oil of bergamot. 



I 



Pass through xylol, and mount in balsam. 



