cover glasses and crushed, after which procedure the 

 covers are quickly drawn apart; or the cover glasses may 

 le drawn over the freshly cut surface of the tissue. Ihese 

 preparations may after fixing be stained after any one of 

 the following methods : 



Haematoxylin and Eosin Stain. 



The preparations are fixed for about 1 hour in ether 

 and alcohol or are exposed to a temperature of 100 to 110 

 C. for about 45 to 60 minutes, stained in Boehmer's hsema- 

 toxylin for 20 to 30 minutes, washed in flowing water, 

 counter-stained in eosin for 5 minutes, again washed, and 

 quickly dried between filter -paper. Mount in balsam. 



All nuclei are stained blue, protoplasm and eosino- 

 phile granules red. The other granules are not stained. 



Ehrlich's Neutropkile Mixture 



Orange G (saturated aqueous solution), . 130-135 c. c. 

 Acid fuchsin (saturated aqueous solution), 80-165 c. c. 

 Methyl green (saturated aqueous solution), 125 c. c. 



Distilled water, '. -:' . t . . . . 300 c. c. 



Alcohol (absolute), ..... 200 c. c. 



Glycerine, . .... . . . 100 c. c. 



Mix orange G, acid fuchsin, water, and absolute alcohol 

 in a bottle, add slowly and while shaking the methyl 

 green. The glycerine is then added. For staining in 

 Ehrlich's neutrophile mixture, the preparations need to be 

 hardened in ether and alcohol for about one hour, or fixed 

 at a temperature of 100 to 110 5 C. for 15 to 30 minutes. 

 Float the preparation on a small quanitity of the stain for 

 about 15 minutes, wash in flowing water, dry between 

 several filter papers, and mount in balsam. The red cor- 

 puscles should have a reddish-brown color (brick color), 

 all nuclei green, the eosin ophile granules red, and the 

 neutrophile granules violet-red. 

 Chenzinski's Stain. 



Methylene blue (saturated aqueous solution), . 40 parts. 

 Eosin (1% sol. in 70% alcohol), ... 20 parts. 



Distilled water, 30 parts. 



Glycerine, 10 parts. 



