172 



The eosin, distilled water,and glycerine are mixed in a 

 bottle; the methylene blue is added slowly while shaking. 

 Preparations need to be fixed from 1 to 1-J- hours at a tem- 

 perature of 120 C. or in ether and alcohol for 1 to 2 hours. 

 They remain in the sta,in for 24 hours in the warm oven at 

 a temperature of 40 C. Wash quickly in flowing water, 

 dry between filter paper, and mount in balsam. The red 

 corpuscles and the eosinophile granules are stained red, all 

 nuclei blue. 



Ehrlich's Triacid Glycerine Mixture. 



Aurantia, ... .. . k -. . , 2 grms. 



Eosin . ;, ... . . .,_ v 3 grms. 



Nigrosin, . v . '. . . - . 5 grms. 



Glycerine, a ....". .. . . 40 c. c. 



The glycerine is divided into three parts, to each is 

 added one of the above stains, and each needs to be ground 

 in a mortar for several hours. The three glycerine solutions 

 are then mixed, and exposed to a temperature of 60 C. for 

 two weeks. The stain is then ready for use, and if well 

 made amply repays all the trouble taken in making.it. It 

 will keep for a long time, and should be of a syrupy con- 

 sistancy^ The blood preparations need to be fixed at a 

 temperature of 130 to 140 C. for 1 to 3 hours. A small 

 quantity of the stain is spread out in a flat dish; on this 

 the preparations are placed, they remain in the warm oven 

 (40 C.) for 24 hours; are then washed in flowing water, 

 dried between filter paper, and mounted in balsam. The red 

 blood cells are stained yellow, nil nuclei black, the eosino- 

 phile granules red, other granules are not stained. 



Ehrlich's Methylene Blue Solution. This is employed 

 for staining basophile cells (Mastzellen of Ehrlich). 

 Methylene blue (saturated alcoholic solution), . 1 part. 

 Distilled water, . . .... 2 parts. 



Preparations are fixed at a temperature of 110 C. for 

 30 minutes. Stain for 15 minutes. Wash quickly in flow- 

 ing water, dry between filter paper, and mount in balsam. 

 All nuclei are stained blue, and only the basophile granules, 

 which also take a blue color, are stained. 



