134: THE AMERICAN MONTHLY [June, 



In alkaline bouillon the growth is very feeble, the liquid becoming 

 barely clouded, even after several days standing. In acid bouillon the 

 growth is at first also feeble, but on the second day it is very much in- 

 creased, the liquid becoming quite clouded, and a granular pellicle 

 forms over the surface. The acid reaction is not appreciably changed. 

 In sterile milk at 36° C. this germ coagulates the casein after about two 

 weeks standing. It grows very vigorously. The reaction remains 

 slightly acid. A slight sour-milk odor is emitted. 



Sterile urine at 36° C. This germ multiplies very rapidly in this 

 liquid. After 18 •hours it becomes quite clouded with the formation of 

 a grayish sediment. Its acid reaction is changed to a feebly alkaline 

 one, which becomes very strong after 36 to 48 hours. The odor is pen- 

 etrating, resembling that emitted V)y ammonia water. In acid bouillon 

 plus urea at 36° C. this germ grows very rapidly. After 24 hours the 

 liquid is very clouded, with considerable sediment formed in the bottom 

 of the tube. The reaction is changed to a strong alkaline one. A 

 slight ammoniacal odor is emitted. In the acid gelatine plus urea the 

 growth is more vigorous and the liquefaction more rapid than in the 

 ordinary gelatine. With the liquefaction its reaction is changed to a 

 strong alkaline one. The liquid gelatine becomes perfectly cleared, 

 with a somewhat viscid sediment. 



This germ is easily stained with the aniline dves. It does not take 

 the Gram stain. 



Micrococcus B. — A micrococcus varying from r.o //. to 1.8 // in di- 

 ameter ; average, 1.4//.. It occurs single, in pairs, and in small clumps. 

 It is occasionally seen in short chains, especially when grown on agar 

 or gelatine. 



Agar (surface growth) at 36° C. — The growth of this germ on agar 

 resembles in its general appearance that of the micrococcus A. It 

 differs from that germ in two respects : (i) The growth on the sui'face 

 is very viscid. (2) The condensation water remains clear, with a 

 grayish, somewhat viscid growth at the bottom. 



Gelatine (needle culture) at 75° F. — After 24 hours a grayish granu- 

 lar line appears along the needle track. At the surface the growth is 

 more vigorous, spreading and liquefying the gelatine in a thin layer 

 over the entire surface. The growth along the needle track does not 

 increase, but the gelatine gradually liquefies from the surface downward 

 until from one-half to two-thirds of the gelatine has become liquid. 

 The liquefaction does not extend beyond that point. A more or less 

 thick, whitish membrane forms over the central portion of the surface, 

 rarely forming a complete pellicle. A layer of grayish, viscid growth 

 forms over the surface of the non-liquefied gelatine. The liquid por- 

 tion holds in suspension grayish granules, which are occasionally so 

 fine as to give a uniform clouded appearance to the liquid gelatine. 

 The liquefaction is completed in from one to two weeks. 



In bouillon at 36° C. the growth is very feeble. After 24 hours the 

 liquid is very faintly clouded. In from two to four days a somewhat 

 stringy growth appears in the upper part of the liquid ; also granular 

 masses, varying from i to 2 millimeters in diameter. These are quite 

 viscid. In acid bouillon, after several days a few granular masses are 

 formed on the surface ; also a whitish deposit on the sides of the tube 

 at the surface of the liquid. 



