1891.] MICROSCOriCAL JOUHNAL. 135 



In sterile milk this crerm, after about two weeks, coagulates the 

 casein, leaving a clear supernatant liquid. 



Sterile urine at 36'^ C. — This gerni grows rather feebly in urine. 

 After 24 hours the liquid is very faintly clouded and neutral in reaction. 

 After 48 lu)urs it is stronglv alkaline and clouded with the formation 

 of a small quantity of sediment. In acid bouillon plus urea the growth 

 is not vigorous. After 34 hours the reaction is feebly alkaline. At the 

 end of two to three days the reaction is strongly alkaline and the liquid 

 (juite cloudy with a slight penetrating odor. In acid gelatine containing 

 urea the growth is more vigorous than in the ordinary gelatine. The 

 liquefied gelatine is strongly alkaline in reaction. 



This germ is easily stained with the aniline dyes. It takes the 

 Gram stain. 



Micrococcus C. — A micrococcus varying in diameter from o.S ,". to 

 1.5 /a; average, 1.2 ;/.. It occurs single, in short chains, and in small 

 clumps. There are also diplococci forms. 



Agar (surface growth) at 36° C. — On this medium the growth does 

 not differ appreciably from that of micrococcus A. 



Gelatine. — This germ liquefies gelatine very slightly, so th<U the 

 growth, which at first is upon the surface, after several days becomes 

 slightly depressed, never more than one to two millimeters. The growth 

 along the needle puncture is whitish, opaque, viscid, and quite thick. 

 The colonies along the needle track are very small. They do not 

 liquefy the gelatine. 



In bouillon the growth is very feeble ; after 24 hours the liquid be- 

 comes faintly and uniformly clouded. There is no further development. 

 Sterile milk inoculated with this germ and kept at a temperature of 36'' 

 C. becomes thickened in the lower half of the tube after about six days. 

 There is no separation of the casein from the aqueous portion ; no odor ; 

 slight acid reaction. 



Sterile urine. — In this liquid the growth is feeble. On the day fol- 

 lowing its inoculation the reaction is faintly alkaline, the liquid barely 

 clouded. On the third dav there is a thin granular deposit on the sides 

 of the tube, and the reaction is strongly alkaline. In acid bouillon plus 

 urea at 36° C, the liquid becomes clouded on the day following the 

 inoculation, with the formation of a considerable quantity of sediment; 

 strong alkaline reaction, penetrating odor. After ten days standing it 

 becomes clear, with a sediment. It remains strongly alkaline. In acid 

 gelatine plus urea the growth is very feeble ; no liquefaction. 



It is easily stained by the ordinary methods. When stained accord- 

 ing to Gram a great majority of the cocci take the brown stain perfectly. 

 A few (about one in fifteen) retain the blue stain. These are of the 

 maximum size (1.5 /*). 



Micrococcus D. — A micrococcus varying in diameter from 0.7 //. to 

 1.2 ,a ; average, 0.9 //., It grows most usually in small clumps and in 

 pairs. It is very rarely seen single. 



Agar (surface growth) at 36° C. — When not crowded the isolated 

 colonies, after 24 hours, are from i to ii^ millimeters in diameter, round, 

 flat, with a smooth border and sharply defined margin ; a considerable 

 growth in the condensation water. The colonies at first are grayish 

 white, glistening, but after several days they become a light cadmium 

 yellow. The size is very slightly increased. When grown on agar 



