Miscellaneous. 149 
On the Regeneration of the Limbs in the Azolotl (Siren pisciformis). 
By J. M. Puiiirravx. 
On the 24th of September, 1866, I had the honour to bring before 
the Academy some experiments demonstrating that the limbs of the 
newt (Triton cristatus) are only regenerated when at least the basal 
part of these members is left in its place (that is to say, the sca- 
pula, when, as in my experiments, we have to do with the anterior 
limbs). It appeared to me necessary to repeat these experiments 
upon other animals of the same class, in order to see whether we 
have to do in this case with a constant fact, as, indeed, everything 
would lead us to suppose. 
By the kindness of M. Duméril, I have had at my disposal ten 
Axolotls bred in the menagerie of reptiles at the Museum of Natural 
History. On the 4th of October, 1866, I removed the left anterior 
limb, including the scapula, from five of these Axolotls ; from the 
five others, on the same day, I amputated the right anterior limb, 
with scissors, at the surface of the body, consequently leaving in 
place not only the scapula, but also the head of the humerus. 
It is now more than eight months since the operation was per- 
formed ; and it is easy to prove that it has furnished the results 
which I had foreseen. In the Axolotls of the first series cicatrization 
has taken place in the most regular manner; but there has not yet 
been the least indication of any regeneration. In those of the second 
series, on the contrary, very soon after the operation, the cicatrix 
began to rise, and there was formed a projection which has gradually 
increased, and I was able to trace day by day the phenomena of the 
regeneration of the limb. Already, and indeed for a long time 
past, this limb has been completely reproduced, with all its normal 
characters of form and structure. 
Thus all the experiments which I have made since I commenced 
studying the question of the reproduction of removed parts. con- 
stantly lead me to the same conclusion. Whether we have to do 
with the removal of entire limbs, as in the Batrachia, or with that 
of more deeply seated organs, such as the spleen in the Mammalia, 
regeneration never takes place except when the operation has left in 
position, and with its normal anatomical connexion, a portion of the 
limbs or of the spleen. This constancy in the results already at- 
tained has encouraged me to try other experiments, the results of 
which I will hereafter communicate to the Academy.—Comptes 
Rendus, June 10, 1867, p. 1204. 
On the Development of the Brown Aphis of the Maple. 
By MM. Bauprani and Signorer. 
The facts recently observed by M. Dareste in the evolution of the 
common fowl, and the deductions which he has drawn from them 
with regard to the production of races in animals, with the conclusive 
analogous examples in plants made known by M. Naudin, demon- 
strate that, in both kingdoms, certain anomalies of development may 
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