How New York City's Health Department Makes 



Preparing the 

 Bacteria 



In the preparation of 

 typhoid vaccine the 

 bacteria are grown in 

 the bottles you see on 

 the table. The germs 

 are killed by pouring a 

 salt solution over them. 

 The bottle on the shelf 

 contains the salt solu- 

 tion which is being si- 

 phoned into the bottle 

 containing the colony 

 of typhoid fever germs 



Transferring the Bacilli 

 Tetanus bacilli occur in dust, earth and 

 manure. They do not grow in the presence of 

 oxygen and are particularly dangerous if they 

 get into deep wounds. The young lady in the 

 photograph is drawing up tetanus bacilli from 

 the bottom of a test tube by means of a 

 tube which she - holds in her mouth. Thus 

 the germs are transferred from tube to tube 



The Resultant Powerful Toxin 



Here the large flask is being inoculated with 

 tetanus bacilli. The small wire basket at the 

 right contains test tubes in which are the 

 tetanus germs. The flame which you see just 

 to the right of the flask which is being inoculated 

 is kept burning so that any instrument may 

 be immediately sterilized after exposure 



The Final Step 



The finished product 

 containing millions of 

 dead typhoid fever ba- 

 cilli is poured by I 

 of a siphon into large 

 bottles. The vaccine is 

 kept in a refrigerator 

 until it is needed for use. 

 Note the milky appear- 

 ance of the vaccine 



