20 



observed on the Rothamsted grass plots, where the unmanured plot 

 carries the largest number of species but a low crop, whilst the 

 heavily manured plots carry a smaller number of species. One 

 organism was described in some detail, Amoeba gobannieusis, 

 which is of interest because it is closely allied to Amoeba cucumis. 

 A Chlamydoplirys was also found, different from the forms pre- 

 viously described. 



NOTE. — In a later paper, which was almost ready for the Press 

 when the authors were called up for military service, Messrs. Lewin 

 and Martin describe another method by which protozoa in the 

 trophic form were picked out of the soil alive. A glass tube 

 \\ inches in diameter and about 2 feet long is closed at the lower 

 end with a rubber bung through which passes a glass tube drawn 

 out to a jet whereby a current of air can be blown through. The 

 tube having been clamped upright, a newly made suspension of the 

 soil in water is poured in until the water level nearly reaches the top. 

 Three hooks are hung round the rim of the tube so as to furnish a 

 support for the cover slip which is smeared with agar jelly and placed 

 about 4 inch above the water level. Air is now blown through the 

 jet so as to produce a stream of fairly small bubbles rising through 

 the suspension and breaking on the cover slip. After about 30 

 seconds the air stream is stopped and the cover slip lifted off and 

 examined under a microscope. 



XIX. A Preliminary Communication on Three new 

 Proteomyxan Rhizopods from Soil." T. GOODEY. 

 "Archiv fur Protistenkunde," 1914. 35, 80—102. 



The organisms described in this paper were obtained in 

 cultures of soil on a nutrient agar medium and the soils which 

 yielded them were all of a rich character. They were first obtained 

 from a cucumber house at Harpenden, and from a vinery in 

 Hampshire ; later on they were obtained from rich garden soils 

 taken in Edgbaston, Birmingham, and Great Barr, Staffordshire. 

 This shows that they are widely distributed. They are large 

 amoeboid forms and are new to science. 



Leptomyxa reticulata nov. gen. et. nov. spec, is the 

 largest of the three and measures from 40 /x to 1 m.m. in greatest 

 breadth. The protoplast spreads out into a thin sheet branching 

 and anastomosing; pseudopodia often filose and delicate; con- 

 tractile vacuoles numerous and minute, multinucleate, each nucleus 

 small and possessing a comparitively large karyosome. Encys- 

 tation into double-walled cysts ; excystation of original organism. 



Leptomyxa flabellata nov. spec. The protoplast is naked 

 and spreads out into a thin sheet frequently resembling a 

 fan in outline, measuring from 60 \i to 150 [x in breadth. The 

 pseudopodia are filose but do not anastomose ; contractile vacuoles 

 numerous and minute. Multinucleate, possessing from 1 to 14 

 nuclei much larger than those of L. Reticulata each having a large 

 karyosome. Encystation into double-walled cysts, the ectocyst 

 showing well defined prominences ; excystation of original 

 organism. 



Gephyramoeba delicatula nov. gen. et. nov. spec. The 

 protoplast is naked and stretches out into long narrow arms 



