BILIIIUM1N. 483 



is evaporated, the residue first freed of fatty acids by 

 treatment with ether, and then of bilirubin, by means 

 of chloroform (bilifuscin purified with ether is insolu- 

 ble in chloroform ; its solubility in chloroform is 

 caused by the presence of fatty acids), then dissolved 

 in alcohol, and this solution evaporated. Almost 

 black, lustrous, brittle mass. Insoluble in water, 

 ether, and chloroform, easily soluble in alcohol, form- 

 ing a solution of a deep-brown color; also in alkalies. 

 With nitric acid it gives the same reaction as bilirubin. 



Biliprasin, C 46 H 22 N 2 6 . Is obtained in a pure con- 

 dition when its alcoholic solution (see above) is evapo- 

 rated, foreign substances removed from the residue 

 with ether and chloroform, the residue redissolved in 

 a little alcohol, and this solution evaporated. Lus- 

 trous, almost black, in pulverized condition greenish- 

 black mass. Fuses when heated, and decomposes, at 

 the same time increasing in volume. Insoluble in 

 water, ether, chloroform ; easily soluble in alcohol, 

 forming a clear green solution. If ammonia be added 

 to this solution, it turns brown (difference between it 

 and biliverdin) ; hydrochloric acid turns it green again. 



Bilihumin. Is contained in considerable quantities 

 in biliary calculi, and is produced from all the other 

 biliary coloring-matters when their solutions in soda- 

 ley are exposed to the air for a long time. Blackish- 

 brown, powdery substance. 



In addition to the coloring-matters described, others 

 occasionally occur in bile. These are, however, unin- 

 vestigated up to the present. 



