1885.] 



MICROSCOPICAL JOURNAL. 



55 



5. Micrometry, exposition of dif- 

 ferent methods. 



6. Cultivating bacteria, exposition 

 of different methods. 



7. Injecting vessels and tissues, 

 demonstration of various methods. 



8. Staining tissues in mass, simple 

 and compound stainings. 



9. Staining vsections, simple and 

 compound stainings. 



10. Section cutting, soft tissues, 

 use of various microtomes. 



11. Section cutting, hard substan- 

 ces, methods of cutting and grinding. 



12. Section cutting, serial sections, 

 S. H. Gage's methods. 



13. Use of dissecting microscope, 

 methods of dissecting, etc. 



14. Practical demonstration of the 

 relation of aperture to power in ob- 

 jectives. 



15. Methods of measuring aper- 

 ture, power, focal length, etc. 



16. Methods of manipulation, de- 

 cantation, desiccation, isolation, etc. 



17. Methods of illumination for 

 special purposes, special objects, etc. 



18. Uses of the mechanical finger, 

 applications to research, etc. 



19. Electrical and thermal appli- 

 cations, uses of hot stage, etc. 



20. Uses of live boxes, growing 

 cells, troughs, compressors, etc. 



21. Special methods of treatment 

 or examination of special subjects, 

 such as blood, pus, sputum, urine. 



22. .Staining and mounting bac- 

 teria, micrococci, etc., for examina- 

 tion. 



23. Special methods of cell mak- 

 ing, cementing, cover cutting, etc. 



24. Special methods of mounting, 

 labeling, finishing, packing, and stor- 

 ing slides, etc. 



All microscopists who expect to 

 attend the Cleveland meeting and are 

 willing to take part in the working 

 session, and assist in the above de- 

 monstrations, are cordially invited to 

 communicate with me on the sub- 

 ject as soon as possible, and any sug- 

 gestions regarding the Working Ses- 

 sion and the subjects to be presented 



and demonstrated there will be very 

 welcome. 



Every indication so far points to a 

 large and successful meeting at Cleve- 

 land, fully equal to any of the preced- 

 ing meetings, and it is hoped to make 

 the working session a valuable fea- 

 ture of the meeting. The active co- 

 operation of microscopists who are 

 interested will insure such a result. 



C. M. VORCE. 



Cleveland, Ohio. 



Culture Media for Bacteria. 



In the Journal of the American 

 Medical Association^ under the head 

 of Foreign Correspondence, a writer 

 in Berlin gives some information con- 

 cerning the preparation of solid cul- 

 ture media. We print the greater 

 part of two letters, as they seem to 

 be written by a person of experience 

 in the culture of micro-organisms. 



The method of preparing the flesh- 

 peptone-gelatin is given as follows : — 



' Haifa pound (0.25 kilo.) of fresh 

 lean meat (beef or mutton) is finely 

 chopped, and to it is added a pint 

 (500 c.c.) of distilled water, the 

 mixture remaining in a cool place 

 for twelve to twenty-four hours. It 

 is then strained through fine linen 

 gauze, the mass being pressed to ex- 

 tract all the liquid, which appears as 

 a reddish-bloody fluid. To this pint 

 of "flesh-water" in a clean flask is 

 added 75 grains (5 grammes) of pep- 

 tone, 30 grains (2 grammes) of com- 

 mon salt, and from i to 2 ounces (30 

 to 60 grammes) of fine gelatin, and 

 to the flask is fitted a cotton-wool 

 stopper. The mixture should then 

 stand for a half hour to allow the 

 gelatin to swell up. and as gelatin 

 has an acid reaction, enough sodic 

 carbonate (Nag CO3) should be added 

 to make the solution neutral or veiy 

 slightly alkaline ; because most germs 

 do not grow in an acid solution. The 

 solution is then placed in a Koch's 

 steam sterilizer and cooked for an hour. 

 This is an apparatus made of tin and 

 covered with thick felt, by which the 



