1885.] 



MICROSCOPICAL JOURNAL. 



107 



portion of 8 to i. 3. Concentrated 

 solution crystals of hematoxylin in 

 alcohol or in No. i , and add a few 

 drops as required to the mixture of 

 I and 2. (Especially recommended 

 for embryos, and very satisfactory.) 



43. AlleyreCook. Note on logwood 



staining solution. Journ. of 

 Anat. and Physiol., vol. xiv, p. 

 140. 



Extract logwood, 6 pts. ; alum, 6 ; 

 copper sulphate, i pt. ; water, 40 pts. ; 

 thymol, a small crystal. Rub the 

 first three well in a mortar, add water 

 to a thin paste, stir occasionally for 

 two days, filter, and add the thymol 

 to preserve it. 



The solution acts on material fresh 

 and hardened in alcohol. For chro- 

 mic acid preparations use eight drops 

 of the above tincture to 1 20 of water 

 and one of a 1% solution of potassium 

 bichromate. For mounting in balsam 

 wash well in absolute alcohol to pre- 

 vent bleaching. 



(Bleaching will occur more or less. 

 The solution of bichromate is too 

 weak to be efiective. The dilution 

 is I to 130,000.) 



44. P. Mayer. Ueber die in d e r 



Zoologischen Station zu Nea- 

 pel gebrauchlichen Methoden 

 zur mikroskopischen Unter- 

 suchung. Mitth. d. Zool. Stat. 

 Neapel, Bd. iv, p. i. 

 Kleinenberg's method is highly rec- 

 ommended and is modified slightly by 

 adding one volume of strongly-concen- 

 trated solution calcium chloride and 

 alum in 70% alcohol to 6-8 of spirit 

 of the same strength. To this is added 

 as many drops as required of a solu- 

 tion of hematoxylin crystals in abso- 

 lute alcohol. 



45. Renaut. Sur le mode de pre- 

 paration, etl'emploidel'c^osine, 

 et de la glycerine hematoxy- 

 lique en histologic. Arch, de 

 Physiol., 1 88 1, p. 640. 

 A thick neutral glycerin is satu- 

 rated with alum, and about one- 

 fourth as much of an alcoholic solu- 

 tion of hematoxylin added drop by 



drop. Too much causes a precipi- 

 tate, and the alumed glycerin must 

 be added to clear up ; filter and set 

 in the light for some weeks, till no 

 smell of alcohol is perceptible ; filter 

 again, and it is fit for use, and will 

 keep well. It dyes in five to ten min- 

 utes. Renaut uses a few drops as a 

 mounting fluid. 



(We find the color leaves the fluid 

 and goes into the preparation, which 

 is therefore very good. At first, how- 

 ever, the uniform color renders the ob- 

 jects less distinct and transparent.) 



46. Dippel, Das Mikroskop, 2 Aufl., 



1882. Bd. i, p. 719. 

 Kleinenberg's method is herein sim- 

 plified. A saturated solution of chlor- 

 aluminium is diluted by 6-8 volumes 

 of 70% alcohol. Alcoholic solutions 

 are recommended. A mixture of al- 

 cohol, alum, and hematoxylin solu- 

 tion diluted with 50-70% alcohol or 

 water is also described. 



47. Friedlander. Mikroskopische 



Technik. Berlin, 1882, p. 43. 



A process similar to Renaut's is 

 here described, which only difl'ers by 

 the use of determined volumes, as 

 follows: Hematoxylin, 2.00; alco- 

 hol, 100.00; distilled water, 100.00; 

 glycerin, 100. o; alum, 2.0 gm. 



(Hematoxylin is very much used 

 in double staining, which see.) 



48. Rindfleisch. 



Uses concentrated watery solutions 

 of hematoxylin and alum. Pour the 

 first into the second. 



o 



The Sizes of Blood -Corpuscles. 



Professor Theodore G. Wormley, 

 in the new edition of his work, gives 

 the following sizes of blood-corpus- 

 cles, as measured by himself and 

 Professor Gulliver. We have only 

 copied the sizes for mammals and 

 birds. It will be seen that, with 

 three or four exceptions, the sizes 

 obtiiined by the two observers are 

 practically the same : 



Mammah. Wormley. Gulliver. 



Man, 1-3250 1-3260 



Monkey, . . . 1-3382 1-34 12 



