1885.] 



MICROSCOPICAL JOURNAL. 



133 



INDIGO-CARMINE. 



6i. Thiersch. Injectionsmassen von 



Thiersch u.W.Miiller. Arch. 



Mikr. Anat. Bd. i. 1865. 

 Make a saturated solution of indigo- 

 carmine in oxalic acid (1:23-30 of 

 water). Dilute with alcohol. When 

 strong gives intense blue in a few 

 hours. Cells and nuclei are colored, 

 and anv excess may be removed by 

 oxalic acid. Introduced into the 

 bodies of living animals indigo-car- 

 mine is taken up by the tissues and 

 again separated. Without attempt- 

 ing to analyze the relations of this 

 process with staining proper, an ab- 

 stract of its literature is given here on 

 account of its importance. 

 63. Chrzonszczewski. (i) Centelbl. 



f. d. Med. Wiss. 1864. No. 



38. (3) Arch, pathol. Anat. 



Bd. XXXV, p. 135. 1866. 

 Chrzonszczewski discovered this 

 method, by introducing colors into 

 the blood of living animals, and 

 studying their excretion by the urinary 

 capillaries. 



63. Diaconow. Medicinisch-chemis- 



cheUntersuchungen. Berlin, 

 1867. p. 245. 

 Indigo-carmine introduced into the 

 stomach or the blood is taken up and 

 then separated by both liver and kid- 

 neys. Other tissues do not absorb it, 

 it is not found in the lymph or serum 

 of the blood. 



64. Heidenhain. (i) Arch. Mikr. 



Anat. Bd. x. p. 30. (2) 

 Arch. f. d. ges. Phys. Bd. ix. 

 p. 1. (3) Hermann. Hand- 

 buch d. Physiologic. Bd. v, p. 



345- 

 Applies the above processes to show 



that the urinary tubuliferi separate 



soluble material and the Malpighian 



glomerules of the kidneys separate 



the water. 



65. Arnold. Arch. f. path. Anat. u. 



Phys. Bd. Ixiv, p. 203 ; Bd. 

 Ixv, p^ 77 ; Bd. Ixvi, p. 77 ; 

 Bd. Ixviii, p. 465 ; Bd. 

 Ixxiii, p. 125; Centralbl. f. 

 d. med.Wiss. 1875, No. 41 u. 

 51. 1875 bis 1878. 



66. Thoma. Centralbl. f. d. med. 



Wiss. 1875 No. 3. Arch. path. 



Anat. u. phys. Bd. Ixiv. p. 



294. 

 67 Kiittner. Arch. t. path, Anat. u. 



Phys. Bd. Ixv, p. 13; Bd. 



Ixvi, p. 13, Centralbl. f. d. 



Med. Wiss. 1875, No. 41. 



68. Gerlach. Centralbl. f. d. Med. 



Wiss. 1875, No. 48. Ueber 

 das verhalten des indigschwe- 

 felsauren Natrons in dem 

 Knorpelgewebe lebender 

 T h i e r e . Habitations-schrift. 

 Erlangen, 1876. 



69. Nykamp. Arch. Mikr. Anat. 



Bd. xiv, p. 493. 



70. Zeller. Arch. path. Anat. u. Phys. 



Bd. Ixxiii, p. 357. 



Nos. 65 to 69 treat of the separa- 

 tion of sodium sulphindigotate in the 

 gelatinous matter between the epithe- 

 lium cells and the walls of the vessel. 

 Nos. 65, 6^^ 69 especially of cartila- 

 ginous tissues. Kiittner examined 

 the separation of the color by the 

 basal portions of pulmonary epithe- 

 lium, and Zeller the same in certain 

 glands of frogs. 



Arnold (in Arch. f. path. Anat. u. 

 Phys. Bd. Ixvi) gives a description 

 of apparatus for the slow injection of 

 colors into the abdominal vein of 

 frogs. 



Sodium sulphindigotate has been 

 employed in double staining, which 

 see. 



[ To be continued.'] 



The Chroinatoscope. 



Some time ago Mr. J. D. Hardy 

 devised an instrument, which he has 

 named a chromatoscope, so easily 

 made by any one who has a spot- 

 lens that we take the following de- 

 scription from the Journal of the 

 Royal Microscopical Society : — ' Its 

 chief purpose is that of illuminating 

 and defining objects which are non- 

 polarizable, in a similar manner to 

 that in which the polariscope defines 

 polarizable objects. It can also be 

 applied to many polarizable objects. 

 This quality, combined with the 



