154 



THE AMERICAN MOKTHLY 



[Angust, 



Sections of bone are stained by an 

 anilin blue that is soluble in alcohol, 

 not in water. The sections are slightly 

 scraped with the scalpel, then put in 

 a warm concentrated alcoholic solu- 

 tion of anilin blue on the water 

 bath, where they remain till nearly 

 dry. They are then rubbed on a fine 

 stone, moistened with a 2% solution 

 of salt, in which they are at length 

 well washed, and finally sealed in a 

 mixture of equal parts of the salt solu- 

 tion and glycerin, 



85. Cornil. Sur la dissociation du 



violet de methyl-aniline et sa 

 separation en deux couleurs 

 sous I'influence de certaines 

 tissus normaux et pathologi- 

 ques, en particulier par les tis- 

 sus en deg^n^rescence amy- 

 loide, Comptes Rendus, 27 

 Mai 1875. 

 An aqueous solution of violet 

 methylanilin is recommended. A 

 special advahtage is, that many tis- 

 sues are finely differentiated by the 

 separation into blue violet and red 

 shades which takes place. In hyaline 

 cartilage, for example, the funda- 

 mental tissue becomes red, the cells 

 and their capsviles violet, likewise the 

 fibrillae of connective and elastic tis- 

 sue and the fibres of elastic cartilage. 

 The stain is unfortunately not perma- 

 nent. In ordinary mediums, as gly- 

 cerin, balsam, turpentine, oil of cloves, 

 and alcohol, it is soon exti'acted from 

 the preparation. Amyloid degener- 

 ations hold the methyl violet much 

 better. They appear of a reddish 

 violet, while the healthy elements 

 dye blue ; they may be kept in glyce- 

 rin. On treatment by acetic acid the 

 red color of the degenerate tissues is 

 more permanent than the blue of the 

 healthy parts, 



86, Hermann. Ueber eine neue 



Tinctionsmethode. Tagebl. 



d. 48 Naturfvers. Graz, 1875. 



p. 105. 



Sections stained with fuchsin are 



soaked in alcohol till no more color 



is extracted. The nuclei only retain 



the dye ; the surrounding pale color is 



much altered. Anilin colors soluble 

 in alcohol, and obtained from rosanilin 

 only, are used, especially fuchsin, 

 which is known in commerce as ruby 

 red. The material should be hard- 

 ened in alcohol and may be first treated 

 with chi'omic acid. A good dye is 

 made by adding 0.25 gm. ruby fuchsin 

 to 20 c. c. alcohol of 96% and about 

 20 c. c, water. The mounts may be 

 of the oi'dinary kind, 



87. Wissowzky, Ueber das Eosin 



als Reagenz auf Hamoglobin, 

 und die Bildung von Blutge- 

 fiissern und Blutkorperchen 

 bei Saugethier- und Hiihn- 

 erembryonen. Arch, f, Mi- 

 krosk, Anat. Bd, xii, pp, 

 479-496, 1876, 

 Eosin and alum, each one part, are 

 dissolved in 200 parts of alcohol as a 

 reagent for hoemoglobin, with which 

 it unites in the red blood discs and 

 colors them orange red. The nuclei 

 and stroma of the blood discs de- 

 prived of their hoemoglobin are no 

 more affected by the dye than the 

 white blood corpuscles. 



88. Lavdowsky. Zur feineren Ana- 



tomic und Physiologic der 

 Speicheldriisen insbesondere 

 der Orbitaldriisen. Arch. Mikr. 

 Anat. Bd, xiii, pp, 359-363, 

 An ammoniacal solution of eosin is 

 preferred to one made with water or 

 alcohol. It should be very slightly 

 alkaline or neutral and so dilute as 

 to barely show color. In this the sec- 

 tions lay for 24 hovirs, and are then 

 exposed to the vapor of acetic acid. 

 This solution stains the lining cells 

 of the stomach red, leaving the basal 

 tissue colorless, but in the peptic 

 glands there is little differentiation. 

 Lavdowsky also made a mixture of 

 picric acid and eosin by adding to an 

 ammoniacal solution of the latter some 

 time made, picric acid till neutralized ; 

 this mixture he called picroeosin. 



89. Dreschfeld. Ueber eine neue 



Tinctionsflussigkeit fur histo- 

 logische Zwecke. Med. Cen- 

 tralbl, 1876, No, 40. On 

 a new staining fluid. Jour, 



