1885.] 



MICKOSCOPICAL JOUKNAL. 



155 



Anat. and Phys., vol. xi, p. 

 181-182. 

 Sections of hardened material are 

 stained by a watery solution of eosin, 

 i-ioooor 1500 distilled water. Treat- 

 ment by absolute alcohol extracts the 

 dye from fresh sections. When laid in 

 the above stain for a minute or a 

 minute and a half, they are put in 

 very dilute acetic acid for a few 

 seconds. Eosin is particularly useful 

 for investij^ations of nervous tissue, 

 for the nuclei and nucleoli of ganglia, 

 and the axis cylinder of nerves which 

 dye red, while the medullary sub- 

 stance remains uncolored, and the 

 connective tissue stains more deeply. 



90. Treitel. Eine neue Reaction der 



Marklialtigen Nei venfasern. 



Med. Centralbl. 1876. No. 



9, p. 147. 

 Treitel used several anilin dyes, in- 

 cluding iodine-violet, fuchsin and 

 anilin blue. He found normal me- 

 dullary nerve matter stains deeply, 

 while degenerate nerves stain feebly, 

 and connective tissue not at all. Prep- 

 arations treated by Miiller's fluid stain 

 well. One drop of a i "0 solution to i 

 c.c. of water stains sections in one min- 

 ute. In this method the nucleus re- 

 mains colorless, also the membrane 

 of Schwann, the axis cylinder is 

 slightly tinged. The continued action 

 of concentrated solutions stains all 

 parts. 



91. Baumgarten. Knorpel, Knochen 



und Anilin farbstofle. Med. 

 Centralbl. 1876. No. 37, p. 



Examines the nature of ossification 

 and cartilage by means of Leonhardt's 

 ink, which is a solution of anilin 

 violet. This is applied to portions 

 of the epiphyses of immature bones 

 treated with wood spirit. The sec- 

 tions lay in the dye 2-10 minutes, 

 then in slightly acidulated water till 

 a decided change from a blue to a 

 violet shade occurs, and are then well 

 washed. The cartilage will now be 

 slightly blue or violet, that which is 

 slightly calcified violet to rose and the 

 formed bone slightly reddish or color- 



less, while the marrow is blue. Sim- 

 ilar results may be obtained by treat- 

 ing with fuchsin and washing in hy- 

 drogen chloride. In this case they 

 must be washed in glycerin or absolute 

 alcohol, not in water. The cartilage 

 will then be reddish blue, that which is 

 partly calcified clear blue, and formed 

 bone red or colorless, and all nuclei 

 carmine. 



92. Ehrlich. P. Beitrjige zur Kennt- 

 niss der anilin fjirbungen, und 

 ihrer verwendung in der Mi- 

 kroskopischenTechnik. Arch. 

 Mikr. Anat. Bd. xiii, pp. 

 263-277. 

 Dahlia is monophenylrosanilin, and 

 is closely allied to parma blue, which 

 is diphenylrosanilin, and anilin blue 

 which is triphenylrosanilin. Most 

 of it is 6nly soluble in alcohol, but a 

 variety soluble in water occurs. A 

 reddish shade is usually preferred. 

 In a neutral aqueous solution animal 

 tissues take an intense color, amyloid 

 substances red, and protoplasm bluish 

 violet. Nuclei stain but little or not 

 at all. Treated with very dilute acetic 

 acid the protoplasm and connective 

 tissue bleaches, the nucleus becomes 

 a bluish violet. The *■ plasma cells' 

 of Waldeyer stain and do not bleach, 

 not even after long treatment by ab- 

 solute alcohol. To stain these cells 

 only, harden in absolute alcohol, and 

 treat with absolute alcohol 50, dis- 

 tilled water 100, glacial acetic acid, 

 12^ dahlia till saturated. Leave sec- 

 tions in dye for 12 hours, dehydrate, 

 mount in balsam. Sometimes mucin 

 cells will stain, also rarely the fat of fat 

 cells. 



Some other anilin colors dye plas- 

 ma cells. They are all soluble in 

 water, and are used with 7^pts. glacial 

 acetic acid and 150 parts 40% alcohol 

 and as much dyestufl'as will dissolve. 

 The following have been used : pri- 

 mula, iodine violet, methyl violet, 

 purpurin, safranin and fuchsin dahlia ; 

 and the first four stain plasma cells, 

 only the rest remaining colorless, 

 while the last two merely color the 

 plasma cells more darkly. Ranvier's 



