216 



THE AMERICAN MONTHLY 



November 



rapidly, and the spindle-shaped fibres 

 stand out clearly, but the prepara- 

 tions are not permanent. 

 134. Norner. Beitrag zur Behand- 

 lung mikroskopischer Prapa- 

 rate. Arch. mikr. Anat.. 

 xxi, 351. 

 Magdala red anilin is highly praised 

 as quick, intense, and difierentiating. 

 May be applied to alcoholic and 

 chrome preparations. Besides ani- 

 mal tissues it is especially recom- 

 mended for plants, and especially 

 the lovs^er fungi. The preparations 

 appear permanent, but are not vet old 

 enough to rely on. 

 I z-^ . Certes. On the processes of col- 

 oring living micro-organisms. 

 Amer. Micr. Journ., vol. 

 iii, p. 224. Sur les procedes 

 de coloration des organismes 

 vivants. Note complemen- 

 taire. Bull. Soc. Zool. 

 France, 1881, p. 21,226. 

 Another method of staining imicel- 

 lular beings. A drop of alcoholic 

 solution of cyanin, Bismarck brown, 

 etc., is placed on a glass slip, and 

 spread. The alcohol evaporates, and 

 when nearly dry put on the drop of 

 water containing the infusoria that 

 will be quickly stained. (See no). 



126. Weigert. Zur Technik der 



Mikroskopischen Bacterien- 

 untersuchung. Arch. Path. 

 Anat. u Phys., Ixxxiv, 275. 

 The best description of the appli- 

 cation of anilin dyes in pathological 

 investigations, and especially as re- 

 lates to micro-organisms, is in Fried- 

 lander's book. 



127. Koch. Die Aetiologie der Tu- 



berculose. Berl. klinische 

 Wochenschrift, 1882, No. 15. 

 Ditto. Mi ttheil ungen des 

 Kaiserl. Gesundheitamtes. 



128. Friedlander. Mikroskopische 



Technik, etc.. Kassel und 



Berlin, 1882. 

 The following process is given by 

 Koch for demonstrating tubercular 

 bacilli : — The Section, or dry prepar- 

 ation, is put for 24 hours in a mix- 

 ture of distilled water 200, concen- 



trated alcholic solution methyl blue 

 10, 10 per cent, caustic potash, 0.2. 

 From this the dark blue sections are 

 put in a concentrated aqueous solu- 

 tion of vesuvin for 15 minutes. 

 Then wash, dehydrate with alcohol, 

 clear up in oil of cloves. Nuclei 

 and micrococci will be brown, the 

 bacillus of tubercle a deep blue. 

 [To be co}i/imie(f.] 



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