1885.] 



MICROSCOPICAL JOURNAL. 



236 



ing processes. Sputa is dried in the 

 usual way, and moistened with a 

 very little dilute potash lye (1-2 drops 

 of the 33 per cent, solution to a 

 watch-glass of water) . The bacillus 

 may now be easily seen with a power 

 of 400-500. To avoid changes, dry 

 the cover-glass again, pass two or 

 three times through a gas flame, then 

 treat with a drop of rather dilute 

 anilin violet, or other anilin adapted 

 to stain nuclei. The bacteria of 

 putrefaction will become intensely 

 blue, but the bacillus of tubercle will 

 remain colorless. 



The numerous processes of 1883 

 cannot yet be brought together and 

 presented here. 



From the text-books on Micro- 

 scopy may be added : — 



131. Beale. How to work with the 



Microscope, 5th ed., 1880, p. 



127. 

 Solferino and magenta are old 

 names for our fuchsin, and they ap- 

 pear to have been much used in 

 England. The dyes are boiled in 

 water, to which a little alcohol is 

 added, 10-15 drops to the ounce. 

 Magenta was recommended by Dr. 

 Roberts in 1863, in Proc. R. Soc, 

 xiv, p. 481, on peculiar appearances 

 exhibited by blood corpuscles under 

 the influence of solutions of magenta 

 and tannin. 



132. Frey. Das Mikroskop und die 



mikroskopische Technik, 7. 

 Aufl., Leipzig, 1881, p. loi. 

 Anilin blue that is insoluble in 

 water but soluble in alcohol, may be 

 made soluble in water by treatment 

 with sulphuric acid, and may then 

 be used in water, or as follows : — 

 Soluble blue 2 eg., water 25 cc, al- 

 cohol 20-25 drops. This fluid is 

 especially to be recommended for 

 those materials that are hardened in 

 alcohol. 



DIFFERENTIATION OF TISSUE ELE- 

 MENTS BY THE REDUCTION OF SIL- 

 VER SALTS, ESPECIALLY SILVER 

 NITRATE. 



Out of the great number of articles 



relating to this method, we select 

 only those methods that have a par- 

 ticular technical or histo; ical interest. 



133. Flinzer. De argenti nitrici 



usu et eflbctu proesertim m 



oculorum m orb is sanandis. 



Diss, 1854, ^^^ Coccius 



gearbeitet. 

 The observation is here recorded 

 that after treatment with lunar caus- 

 tic a precipitate is formed between 

 the cells of the cornea. 



(After V. Recklinghausen See No. 

 138.) 



134. His. Beitrage zur normalen 



und pathologischen Histologic 

 der Cornea. Basel, 1856. 

 On treatment of the cornea with 

 lunar caustic a granular precipitate 

 forms in the canals, or in the funda- 

 mental tissue. He calls the first in- 

 tercellular, the latter extracellular. 

 He applies the pencil to the cornea. 



135. V. Recklinghausen. Eine 



Methode mikroskopische 

 hohle und solide Gebilde von 

 einauder zu scheiden. Archiv 

 pathol. Anat. xix, 451. 

 Fresh or dried animal tissues are 

 put into a weak solution of silver ni- 

 trate, then in a dilute brine in order 

 to arrest the further action of light. 

 A fine, thick, black silver precipitate 

 is thus formed in all parts containing 

 much water, while portions more 

 solid escape the feeble action of the 

 silver salt and remain colorless, or 

 with longer treatment show scattered 

 grains, or difl'use staining. 



(The best method of staining with 

 silver is to lay the material in a |- to 

 ^% solution of a silver salt for 20 to 

 40 seconds, moving it about in the 

 solution, taking care that sections 

 do not cling together. Then drop 

 at once in a .75% salt solution, mov- 

 ing them actively as before, then ex- 

 pose to the light). 



136. V. Recklinghausen. Die 



Lymphgefiisse und ihre 

 Beziehnng zum Bierdege- 

 webe. Berlin, 1862. p. 5. 

 The results of continued experi- 



