1899] MICROSCOPICAL JOURNAL. 161 



tation tubes, flasks, Petri dishes and reagent bottles. Put 

 the slides and cover-glasses in the cleaning mixture, they 

 can be rinsed and wiped later. 



Test Tubes. Wash these carefally with soap and water 

 using the test tube brush. After washing stand them in 

 a glass jar (aquarium) and fill them to within about 2 cm. 

 of the top with cleaning mixture. After it has acted for 

 from 4 to 10 minutes (it is better to stand longer) pour it 

 out of the tubes into the bottle originally containing it. 

 Rinse the tubes thoroughly in tap water until all color 

 disappears and then in hot water and drain them, using in- 

 dividual drainage board from locker. After they are dry, 

 wipe the outside of the tubes with a slighty dampened 

 cloth. 



Fermentation tubes. Treat these in the same manner 

 as the test tubes excepting in the use of the brush which 

 must be omitted. 



Flasks. Wash the flasks thoroughly with soap and 

 water. Then fill them with the cleaning mixture and al- 

 low it to act for at least 10 minutes after which it can be 

 poured back. Rinse the flasks thoroughly in the same 

 manner as the test tubes and drain them. When dry the 

 outside should be wiped with a damp cloth, 



Petri dishes and reagent bottles. Thoroughly wash the 

 Petri dishes and reagent bottles in hot soap suds after 

 which rinse them separately in hot water. Wipe the Petri 

 dishes dry with a cloth and drain the bottles. The clean- 

 ing mixture need not be used. 



Slides and cover-glasses. Drop the cover-glasses singly 

 into a glass jar containing cleaning mixture and allow 

 them to remain there for 24 hours or longer. Pour ofi" 

 the cleaning mixture and rinse the cover-glasses until all 

 of the color disappears, then cover them withalcohol un- 

 til needed when they can be wiped with a soft linen cloth 

 or with lens paper. Treat the slides in the same manner 

 as the cover-glasses. They can be wiped directly out of 

 the rinsing water. The slides can be cleaned satisfac- 

 torily by washing them in a strong, hot soap suds, rinse 

 iu water and wipe. 



Cleaning and culture apparatus. Place the tubes, flasks, 

 or Petri dishes rontaininsr old cultures in a water bath, 



