226 tHB AMBRICAK MONTHLY [July 



Staining Tissues. — Picro-anilin-blue is used for lympha- 

 tic g-landular tissue and for nervous tissues. Five cc. of 

 the saturated aqueous solution of anilin blue are added to 

 lOOcc. of a saturated aqueous solution of picric acid. Let 

 the tissue remain but a few minutes in the combined solu- 

 tion. It is often better to use the solutions separately. 

 Allow the tissue to remain in the blue solution until it as- 

 sumes a pale sky-blue color ; then immerse it in the picric 

 solution for ten or fifteen minutes. The nuclei will have 

 stained a brig-ht g-reen, and the surrounding- tissue a pale 

 pea-gfreen. Like results are obtained in both fresh and 

 "fixed" tissues. If the sections are to be mounted in bal- 

 sam the picric stain is apt to be dissolved out by the alco- 

 hol during the process of dehydration. This can be over- 

 come by dehydrating- in alcohol in which picric acid has 

 been dissolved. If the separate solutions have been used, 

 the tissue may be carried direct from the blue stain to a 

 one-half per cent alcoholic solution of picric acid, thus 

 shortening the process. 



BACTERIOLOGY. 



Tuberculosis Among the Queen's Cows. — A tuberculin 

 test was recently madeat Windsor of a herd of forty short- 

 horn and Jersey cows belonging to the Queen, and most of 

 them were found to react to the test. They were all appar- 

 ently in good condition but as a result of the test, thirty- 

 two cows appeared to be tuberculous, their temperature 

 rising- to 104 deg-rees F. or more ; five cows appeared to be 

 healthy, and three were doubtful. The whole herd was 

 killed and the carcasses were examined at the Royal Vet- 

 erinary College. Of thirty-four animals whose tempera- 

 ture had risen above 104 deg-rees F., thirty-three were 

 found to be tuberculous. The remaining- animal was not 

 tuberculous, but had a diseased uterus. The rise in this 

 case was sudden, and did not occur until after the twelfth 

 hour. Of four cows which did not react, three were found 

 to be free from tubercle, and the fourth had one small 

 caseous gland in which tubercle bacilli were found. The 



