30 METHOD OF DESTROYING ALG.E IN WATEK SUPPLIES. 



Effect of copper sulphate upon Bacillus typhi at different temperatures. 

 [Determination made in tubes of bouillon. + indicates growth after 48 hours' incubation; - indi- 

 cates no growth.] 



a Experiment conducted in test tubes, each containing 5 cc. of sterilized water, portions of which 

 had been previously treated with the desired amounts of copper sulphate. All tubes inoculated with 

 a 3 mm. loop of a 24-hour culture of B. typhi. 



Effect of copper sulphate upon Bacillus typhi cultures of various ages. a 



[Determination made in tubes of bouillon. + indicates growth after 48 hours' incubation; indi- 

 cates no growth.] 



Experiment conducted in test tubes each con taining 5 cc. of sterilized water, portions of which had 

 been previously treated with the desired amount of copper sulphate. All tubes inoculated with a 

 3 mm. loop of a culture of B. tyjthi of the proper age. 



Effect of copper sulphate on Bacillus typhi at different temperatures. a 

 [Determination made in Petri dishes.] 



a Experiment conducted in test tubes each containing 5 co. of sterilized water, portions of which 

 had been previously treated with the proper amounts of copper sulphate. All tubes inoculated with 

 a 3 mm. loop of an 18-hour culture of B. typhi. 



