EFFECT OF COPPER UPON PATHOGENIC BACTERIA. 



31 



Effect of copper sulphate upon Bacillus typhi at room temperature.** 

 [Determination made in Petri dishes.] 



Experiment conducted in test tubes each containing 5 cc. of sterilized water, portions of which 

 had been previously treated with the desired amounts of copper sulphate. All tubes inoculated with 

 a 3 mm. loop of an 18-hour culture of B. typhi. 



Effect of copper sulphate upon Bacillus typhi at room temperature." 

 [Determination made in Petri dishes.] 



a Experiment conducted in 12-liter aquaria. No. 1 was untreated; copper sulphate was added to 

 Nos. 2, 3, 4, and 5. Three cubic centimeters of a mixture of cultures of B. typhi were added to each 

 jar 18 hours before treating. All small nonliquifying colonies counted as typnoid. 



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