1898.] MICROSCOPICAL JOURNAL 7\) 



octagonal and ribbed and each segment is bounded by an 

 extended spinulose rib on each side. The outer part is 

 colorless and is gradually colored until a beautiful pink 

 center is reached. 



Mounting in Glycerine Jelly. — ^To mount in balsam 

 the object must be free from moisture, but not so with 

 glycerine jelly. To mount in this medium first soak the 

 object for twenty-four hours in a mixture of equal parts 

 of GWA — glycerine, water and alcohol. Remove the 

 specimen from this mixture, place it upon the center of a 

 glass slip and remove the surplus fluid with blotting 

 paper. Place the bottle of jelly in a pail or cup of 

 warm water until it is entirely limpid. It will then 

 remain limpid for a whole evening. With a small 

 spatula take a drop of the limpid jelly from the bottle, 

 cover the specimen and drop enough on the glass slip to 

 fill the space between slip and cover. It is well to 

 mount in a shallow cell and in such case, fill the cell 

 with the jelly. If after the cover glass is placed the 

 mount be full of bubbles discard it. Bubbles may be 

 removed by boiling the jelly upon the slide. In boiling, 

 use a clip and hold the slide over the flame. It will first 

 begin to bubble from the center outward and soon a per- 

 ceptible crack will be heard. At this moment, quickly 

 withdraw the slide and place it upon a cold surface. 

 Clean it from superfluous jelly with a soft tooth-brush 

 under running water. Then seal with a good cement 

 and ring to suit the taste. 



Balsam Mounting. — No more balsam should be used 

 than sufficient to reach to the edges of the cover-glass, 

 and if this point be carefully attended to, the slip will 

 require no clearing preparatory to finishing. Before 

 using the glass slip, thoroughly clean with alcohol. On 

 the center of the glass slip place a tiny drop of balsam, 

 and with a pair of tweesers place the cover-glass over it, 

 and hold over a spirit lamp until a sea of bubbles is seen 



