No. 4.] REPORT OF CATTLE BUREAU. 309 



inoculatious, as a very fresh and active virus was desired. The 

 virus used was obtained of a vaccine establishment, and was sent 

 sealed in a sjlass tube. This seal was broken and a portion of the 

 virus was drained into a sterile test tu]»e and plugged with sterile 

 cotton. This portion was used in all the subse(iuent experinientAS. 

 Nothing was introduced into this except a sterile glass pipette, 

 anil the techni(|ue ordinarily employed with pure cultures of bac- 

 teria was always observed in handling this tube of virus. Except 

 at times when it was being used for the inoculations, this virus 

 was kept in a refrigerator. 



The experiments may be outlined as follows : — 



Calf No. 1. — Procured July "21, of Mr. Hone of North Suugus. 

 Vaccinated July 31. Sickened and died August 4. Death, four- 

 teen days after purchase. 



Calf No. 2.— Procured July 23, of the Town Farm, Wakefield. 

 Vaccinated July 31. Sickened and died Augusts. Death, thir- 

 teen days after purchase. 



Calf No. 3. — Procured July 23, of Mr. Jesse Edmunds, Wake- 

 field. Vaccinated July 31. Sickened and died August 5. Death, 

 thirteen days after purchase. 



The above three calves were fed on fresh milk and boiled 

 Blatchford's food from the time of purchase to the time of death. 

 Some of the milk was procured from a neighboring farm, the rest 

 from G. R. Tyzzer. They were all vaccinated on July 31, four or 

 five days before their death. Animals recovered from the anaes- 

 thesia, and the vaccine lesions were developing in all cases. From 

 the time of their purchase up to twenty-four hours before their 

 death the calves seemed to be doing well and thriving. 



Calf No. 4. — Procured August 4, of G. R. Tyzzer. This calf 

 had been kept in stable below for three days previous to purchase. 

 Was not vaccinated. Sickened and died August 7. Death, three 

 days after purchase, and about six or seven days after birth. 



Calf No. 5. — Procured August 4, of Mr. Strong, Wakefield. 

 This calf was not vaccinated. Sickened and died August 9. 

 Death, five days after purchase. 



These two calves (Nos. 4 and 5) were fed a larger amount of 

 fresh milk and a smaller amount of Blatchford's meal, and, as has 

 been noted, were not vaccinated. They were, however, kept in 

 the same places as calves Nos. 1, 2 and 3, and were fed from the 

 same pails. 



The train of symptoms preceding death was practically identi- 

 cal in all the five calves. In calves Nos. 1, 2 and 3 vaccine 

 lesions developed at the various points inoculated. In calf No. 1 

 there appeared, on the border of an inoculation made on the edge 



