312 BOARD OF AGRICULTURE. [Pub. Doc. 



the inner border of the vaccine lesion of the lip in all three calves. 

 This was similar in every respect to the lesion observed on the lip 

 of calf No. 1, vaccinated July 31. 



One of these vesicles was ruptured, and the fluid was caught 

 on a cloth which was then rubbed in the mouth of cow No. 3. Jn 

 thirty-six hours there was a rise of temperature, which was fol- 

 lowed in forty-eight hours by lesions in the mouth and a vesicular 

 eruption on the udder and teats. Vesicles developed later in the 

 cleft of hoof. The condition of the mouth and feet was identical 

 with that found in G. R. Tyzzer's cows. 



The infectiousness of the disease was established by its appear- 

 ance in the other two cows (Nos. 1 and 2) without their being 

 inoculated. 



There seemed after these experiments no question but that the 

 disease originated from the vaccine lymph used in the inoculation. 

 It also seemed probable that in the calves there was, besides vac- 

 cinia, a second disease, which became separated in cow No. 3 

 when inoculated with the fluid of the vesicle which developed on 

 the edge of the lip vaccination of the calf. 



Whether or not vaccinia thus presented itself in an unusual 

 form, or whether it was present conjointly with another disease in 

 the case of the cows, was a possibility which it was necessary to 

 consider. To this end lesions were excised from the cows, and a 

 microscopical study made ; and lymph collected from the vesicles 

 was used to inoculate rabbits, in order to determine the possible 

 presence of vaccinia. The results of these inoculations and the 

 appearance of the lesions excised are given in the following 

 section : — 



Pathological Findings in the Experiments carried on at the 



Newcomh Estate. 



On account of the occurrence of a vesicular eruption on the bags 

 and teats of the cows, which cows subsequentl}' developed vesicles 

 of the feet, the possibility of the co-existence of vaccinia with 

 another disease was provisionally entertained. The gross appear- 

 ances of the vesicles and pustules on the bag of cow No. 5 were 

 suggestive of cow-pox. 



In order to determine the presence or absence of vaccinia in the 

 processes observed, three lines of investigation were followed : — 



1. The histological study of the skin lesions. 



2. The inoculation of corneas with Ij'mph from various lesions. 



3. The vaccination of cows that luid recovered from the disease, 

 in order to find out Avhether the said disease had conferred immu- 

 nity to vaccinia. 



