314 BOARD OF AGRICULTURE. [Pub. Doc. 



pustules of various sizes, which, from their position and character 

 of the necrosis present, may have arisen from vesicles similar to 

 those just described. The lesions from cow No. 5, which were 

 supposed to be cow-pox vesicles, gave no evidence of that disease. 

 Large numbers of cocci are present. 



In summary of the above lesions, it is to be noted that they in 

 no instance contain anything typical of the vaccine process. Fur- 

 thermore, it is impossible that pustules such as were found in case 

 of cow No. 5 could have arisen from vaccine lesions in the time 

 given, and they afford no evidence of that process. 



Corneal Inoculations in Rabbits. 



Experiment No. 1: Sept. 4, 1903. — Shallow incisions were 

 made in the corneas of two rabbits, and lymph obtained from the 

 foot vesicles of cow No. 3 was rubbed in. Following this inocu- 

 lation there appeared no gross sign of inflammation, and the 

 cornea became apparently normal. The rabbits were killed three 

 days after inoculation. Microscopical examination showed in each 

 cornea the repair of a simple injury. 



Experiment No. 2: Sept. 8, 1903. — The corneas of two rab- 

 bits were inoculated with lymph from vesicles on the udders of 

 cow No. 3. There appeared subsequently no inflammation in 

 the eyes of either rabbit. One was killed thi'ee days and the 

 other eight days after inoculation. On histological examination, 

 all four corneas were found to be undergoing repair to a simple 

 injury. 



Concerning the results of the two experiments just noted, it 

 should be understood that when the rabbit's cornea is inoculated, 

 either with active vaccine lymph or with small-pox virus, a charac- 

 teristic lesion is produced, which contains large numbers of the 

 vaccine organisms, Cytoryctes vaccinice. Inasmuch as this lesion 

 is very constant, and forms a delicate test for the presence of vac- 

 cine virus in every form thus far met with, it seems improbable 

 that either the fluid of the udder vesicles or the fluid of the foot 

 vesicles contained vaccine. 



Experiment No. 3 : Sept. 18, 1903. — The corneas of calf No 7 

 were inoculated with material from the vesicles of the bag of cow 

 No. 5. This was followed by inflammation in both eyes. There 

 was considerable opacity and loss of corneal substance. The 

 calf was killed three daj's after inoculation. Examined histolog- 

 ically, there was a considerable degree of a^dema of the cornea as a 

 whole. Masses of staphylococci were found in the tissues at the 

 base and edges of the ulcer. There is no evidence of vaccinia, 



