1!K)(J.] PUBLIC DOCUMENT 



Xo 



o n 



• no. 



127 



ized soil. Results of a similar nature have been observed 

 by us a number of times in an incidental way when utiliz- 

 ing sterilized soils in our general experimental work in the 

 greenhouse. The seeds Avere planted in boxes 18 by 12 by 

 3 inches, and previous to planting the soil in the sterilized 

 boxes was heated by steam to a temperature of about 212° 

 F. for one hour. The soil used constituted a good typical 

 loam, charactei'istic of this region, and the sterilized and 

 unsterilized soils were identical in every way except as to 

 steaming. Other conditions, such as heat, light, degree of 

 moisture, etc., were made the same as far as practicable. 

 In Nos. 15 to 23, inclusive, 600 seeds were used in three 

 separate experiments, where 100 seeds were sown in ster- 

 ilized soil and 100 in unsterilized soil. In Nos. 25 to 

 34, inclusive, 800 seeds each Avere employed, there being 

 two experiments. No. 39 is the result of only one experi- 



I'fcblc showing the Gcnninalion of Seeds in Sterilized and Uiislcrilized 



Soil. 



