IO 



15th it was fed ten worms daily. At the same time from the first 

 feeding, a little less than seven days, it had the gapes. It was 

 killed in eight days and twelve gape worms found, all in the 

 trachea. 



Exp. 3. November 13th three chicks, two days old, were fed 

 earth worms from my garden, eight, nine and ten days, respect- 

 ively. No symptoms of the gapes were produced, but to deter- 

 mine positively, the one fed nine days was killed, and no gape 

 worms found. This experiment shows that all earthworms do 

 not contain the embryo of Syngamus. To confirm this, earth- 

 worms from the same place have been repeatedly examined with 

 the microscope, and none of the embryos found. 



Exp. 4. Two mature Syngami were broken in pieces, so as 

 to free the eggs. They were then placed on the surface of a dish 

 filled with dirt, well moistened with water. After two weeks 

 some earthworms were placed in this dish and allowed to remain 

 ten days. Three of these were fed to a chick, which was care- 

 fully watched for two weeks. No symptoms of gapes were dis- 

 covered. Evidently the embryos had not obtained access to 

 the earthworms in sufficient numbers to produce the disease in 

 chicks. 



Exps. 5 and 6. On December 13th, two chicks, four weeks 

 and four days old, were each fed six earthworms from the infected 

 spot, with the same precautions as before. On the 14th, 15th 

 and 1 6th the feeding of six worms was repeated, making twenty- 

 four to each chick. On December 20th, about seven days, as 

 before, they had the first symptoms of the gapes. One was now 

 killed, and twenty-two Syngami were found. On December 

 24th, eleven days from the first feeding, the other was killed, and 

 sixteen f6und. All of them were united, and in the trachea. 

 None were found in the lungs, but it is probable they were there, 

 and want of experience in the search prevented their discovery. 



Exps. 7, 8 and 9. On April 21st, 1884, fed three chicks, two 

 days old, each five worms from the same place where the others 

 were obtained. Repeated the feeding on April 22d, 23d, 24th, 

 25th, 26th and 27th. On April 28th, about seven days from the 

 first feeding, all had the gapes. One was now killed ami Syngami 

 found in the trachea, also three pairs in the lower part of the left 

 lung and one pair in the lower part of the right lung. Continued 

 to feed the two remaining chicks earthworms until May 5th, just 

 two weeks from the first feeding, when one was killed and the 



