CONTENTS. 



CHAPTER I. 

 GENERAL MORPHOLOGY AND BIOLOGY. 



PAGE 



INTRODUCTORY Terminology Structure of the bacterial cell 

 Reproduction of bacteria Spore formation Motility 

 Minuter structure of the bacterial protoplasm Chemical 

 composition of bacteria Classification Food supply Re- 

 lation of bacteria to moisture, gaseous environment, tempera- 

 ture, and light Conditions affecting bacterial motility 

 Effects of bacteria in nature Methods of bacterial action 

 Variability among bacteria . . . . .1 



CHAPTER II. 

 METHODS OF CULTIVATION OF BACTERIA. 



Introductory Methods of sterilisation! Preparation of culture 

 media Use of the culture media Methods of the separation 

 of aerobic organisms Principles of the culture of anaerobic 

 organisms Miscellaneous methods General laboratory 

 rules . . . . . . . . .26 



CHAPTER III. 

 MICROSCOPIC METHODS. 



The microscope Examination of hanging-drop cultures Film pre- 

 parations Examination of bacteria in tissues The cutting 

 of sections -Staining principles Mordants and decolorisers 

 Formulae of stains Gram's method and its modifications 

 Stain for tubercle and other acid- fast bacilli Staining of 

 spores, capsules, and flagella The Romanowsky stains . 92 



