464 CHOLERA 



growth does not take place, but on incubation at a temperature 

 of from 30 to 37 C., a moist layer appears, which assumes a 

 dirty brown colour, somewhat like that of the glanders bacillus ; 

 the appearance, however, varies somewhat with different varieties, 

 and also on different sorts of potatoes. 



In bouillon with alkaline reaction the organism grows very 

 readily, there occurring in twelve hours at 37 C. a general 

 turbidity, while the surface shows a thin pellicle composed of 

 spirilla in a very actively motile condition. Growth takes place 

 under the same conditions equally rapidly in peptone solution 

 (1 per cent, with '5 per cent, sodium chloride added). 



In milk also the organism grows well, and produces no 

 coagulation nor any change in its [.'appearance, at least for 

 several days. 



A B 



FIG. 139. Colonies of the cholera spirillum in a gelatin plate 

 three days' growth. A shows the granular surface, liquefaction just 

 commencing ; in B liquefaction is well marked. 



On all the media the growth of the cholera spirillum is a 

 relatively rapid one, and especially is this the case in peptone 

 solution and in bouillon, a circumstance of importance in relation 

 to its separation in cases of cholera (vide p. 472). 



The cholera organism is one which grows much more rapidly 

 in the presence of oxygen than in anaerobic conditions ; in the 

 complete exclusion of oxygen very little growth occurs. 



Cholera-Red Reaction. This is one of the most important tests 

 in the diagnosis of the cholera organism. It is always given by 

 a true cholera spirillum, and though the reaction is not peculiar 

 to it, the number of organisms which give the reaction under 

 the conditions mentioned are comparatively few. The test is 

 made by adding a few drops of pure sulphuric acid to a culture 

 in bouillon or in peptone solution (1 per cent.) which has been 

 incubated for twenty-four hours at 37 C. ; in the case of the 



