LEISHMANIA DONOVANI 



669 



taking on the bright red colour of nuclear matter when stained 

 by the Romanowsky combination. The larger stains less 

 intensely than the smaller, is round, oval, heart-shaped, or 

 bilobed, and lies rather towards the periphery of the body in 

 the region of the "hinge" in the cockle-shaped individuals. 

 The other chromatin body is usually rod-shaped, and is set 

 perpendicularly or at a tangent to the larger mass, with which 

 only exceptionally it appears to be connected. Usually the 



* SI 



FIG. 190. Leishman-Donovan bodies from spleen smear, x 1000. 



protoplasm contains one or two vacuoles. Though in spleen 

 smears many free bodies are seen, the study of sections shows 

 that ordinarily their position is intra-cellular, the cells con- 

 taining them being of a large mononuclear type (Fig. 191). The 

 view held is that on their entering the circulation they are 

 taken up by the mononuclear leucocytes and by such cells as 

 the endothelial lining of the splenic sinuses or those lining 

 capillaries or lymphatics, that in these cells multiplication takes 

 place, it may be to such an extent as to rupture the cell, and 

 that if thus the bodies become free they are taken up by other 



