CONTENTS. 



PAGE 



Introduction with Historical Sketch . i 



PART I. 

 BACTERIOLOGICAL TECHNIC. 



CHAPTER I. 



Examination of Bacteria with the Microscope, Including Methods of 



Staining 18 



CHAPTER II. 

 Sterilization 53 



CHAPTER III. 

 Culture-media 64 



CHAPTER IV. 



The Cultivation of Bacteria. Tube-cultures; the Incubator; Anaerobic 



Methods 78 



CHAPTER V. 



The Cultivation of Bacteria (Continued). Isolation of Bacteria; Plate- 

 cultures 94 



CHAPTER VI. 

 Inoculation of Animals. Autopsies; Collodion Sacs 102 



CHAPTER VII. 

 Collection of Material 107 



CHAPTER VIII. 



Systematic Study of Species of Bacteria. Suggestions for Class-work; 



Rules 112 



ix 



