EXAMINATION OF BACTERIA WITH THE MICROSCOPE. 39 



re to be made. Sections of exquisite thinness are possible 

 rith this form of imbedding. The knife need not be wet. 

 In order to mount the sections, proceed as follows: 

 (a) Place the sections on water in a porcelain capsule. 

 Varm slightly, when the sections will flatten nicely. Smear 

 le surface of a slide with a very thin layer of Mayer's glycerin- 

 [bumen mixture. Dip the slide under the sections; raise it up, 



FIG. 10. Schanze Microtome. 



and drain off the water, leaving the sections adhering to the 

 glass in their proper positions. Let them dry for some hours 

 in the incubator, and they will be firmly fastened to the slide. 



(b) Dissolve out the paraffin in one of the numerous solvents 

 (xylol, 'a few minutes). 



(c) Wash off the xylol with absolute alcohol, and 



(d) Stain the sections as desired. 



(e) Dehydrate in absolute alcohol. 



