CHAPTER VI. 

 INOCULATION OF ANIMALS. 



IN the study of pathogenic bacteria, the inoculation of animals 

 is frequently indispensable. It is inexpedient where classes 

 are large for students to make such inoculations; but, never- 

 theless, every student should be familiar with the subject. 

 The animals most often used are white mice, guinea-pigs, 

 rabbits and pigeons. Larger animals are also employed for 

 special purposes. The hair in all cases should be removed 

 from the point selected for inoculation, and if the material 

 to be introduced is solid or semisolid. as with tissue pulp, a 

 small V-shaped opening in the skin is made with scissors, and 



FIG. 36. Mouse-holder. 



a stiff, sterilized, platinum wire or with the point of the scissors 

 or of a pair of forceps, is passed into this opening, separating 

 the skin from the muscles for some distance so as to make a 

 pocket. Into this pocket the material is introduced by means 

 of the platinum wire. The wound may be covered with collo- 

 dion. Such solid or semi-solid material may also be conveni- 

 ently introduced by placing them in a sterile glass cannula, 

 which is pushed to the proper situation through a small incis- 



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