IMMUNITY. 205 



Very thin slices one each from near the fissure of Rolando, from Ammon's 

 horn, and from the cerebellum are cut at right angles to the surface taking the 

 gray matter. These are spread upon scrupously clean slides, and rubbed over 

 in one direction gently with cover-glasses. The smears are allowed to dry in the 

 air, and stained by Giemsa's or by Mallory's methods. Giemsa's stain 

 as last recommended consists of: Azure II 0.8; eosin 3.0 gr.; glycerin 

 (chem. pure) 250 c.c.; methyl alcohol (chem. pure) 250 c.c. Both glycerin 

 and alcohol are heated at 60 C. The dyes are put into the alcohol and the 

 glycerin is added slowly, stirring. The mixture is allowed to stand at room 

 temperature over night, and is ready for use after filtering. 



Luzzani* reports the results of himself and those of others 

 in regard to the presence of Negri bodies. This report shows 

 that the bodies were not found in 9 out of 296 cases in which 

 hydrophobia diagnosis was confirmed by inoculation. In the 

 other cases it was found either in sections or smear preparations. 



Marie, f contrary to the usual experience, found that the blood 

 of animals affected with rabies may be virulent under cir- 

 cumstances not yet determined. 



Di Vestea J found that the virus of rabies, which is well pre- 

 served by glycerin in the nerve-tissue, is not so preserved when 

 filtered through unglazed porcelain. In fact, the virus after 

 nitration is in all respects much more labile than when not 

 filtered. Di Vestea comes to the conclusion from this ob- 

 servation that only the young forms of the parasite pass 

 through the filter. Moreover, that the results with glycerin 

 as a preservative are in favor of the idea that rabies virus is 

 intracellular, probably since the glycerin kills the young para- 

 sites which have not yet entered the brain cells, but not those 

 contained in the brain cells. 



Remlinger found that centrifugalizing rabies virus for one 



*Arch. per le sc. med. T. XXVIII., No. 34, pp. 521-540. Abstract in Bulle- 

 tin de I'lnst. Pasteur. Vol. III., No. 7. April 15, 1905. pp. 297-298. 



fCompt. Rendus Soc. Biologic, T. LVIII. 25 Mars, 1905, p. 544. Abstract 

 in Bui. de I'lnst. Past. Vol. III., No. n. June 15, 1905. p. 461. 



Comm. Ac. Medic. Pise, 24 Jan., 1905. p. 2. Abst. Bui. de I'lnst. Pasteur. 

 T. III., No. 8. 30 Avril, 1905. p. 338. 



Compt. Rendus Soc. Biologic. T. LVIII., 7 Janv., 1905. p. 27. Abst. 

 Med. de I'lnst. Pasteur. T. III., No. 7. 15 Avril, 1905. p. 301. 



