300 MANUAL OF BACTERIOLOGY. 



him by Buxton. The streptococcus was grown in beef-broth 

 for ten days and the culture then inoculated with B. prodigiosus 

 and the mixed culture grown for another ten days. These cul- 

 tures, sterilized at 60 C., were injected into the tumors without 

 filtering. 



Streptococcus of Erysipelas. The cause of erysipelas is 

 a streptococcus which in all essential respects in its mor- 

 phology, its growth on culture- media, its behavior with stains 

 and its pathogenic properties corresponds to the Streptococcus 

 pyogenes. It is probable that these organisms are identical, 

 though the clinical manifestations in erysipelas are sufficiently 

 characteristic to justify the clinician in making a distinction 

 between this on the one hand, and the other manifestations of 

 streptococcus infection on the other. In erysipelas, conta- 

 giousness is a most marked feature. 



Micrococcus Tetragenus. Found in the cavities in the 

 lungs of pulmonary tuberculosis, in sputum and in pus. The 

 micrococci are enclosed in a transparent capsule, best seen in 

 preparations from the tissues of inoculated animals, and are 

 arranged in pairs or in fours; about i ^ in diameter; not motile; 

 stain by Gram's method. It grows well at the room tempera- 

 ture, but rather slowly; is a facultative anaerobe; does not 

 liquefy gelatin. Gelatin plates show little, white, punctiform 

 colonies, which, with the low power, are finely granular, and 

 have a peculiar glassy shimmer; in stab-cultures the growths 

 appear as little colonies along the line of puncture. On agar, 

 round white colonies form, which do not tend to spread. It 

 produces a thick, slimy film on potato and a broad, white, 

 moist growth on blood-serum. This organism is only oc- 

 casionally found in pus. It is pathogenic for white and gray 

 mice and guinea-pigs, jbut not for rabbits. It may produce 

 septicemia or only a localized suppuration in guinea-pigs. In 

 white mice general septicemia results on inoculation and the 

 Micrococcus tetragenus is found in the blood and in the great 



