43 



MANUAL OF BACTERIOLOGY. 



TRANSIENT, a few days. 



TURBID, cloudy with flocculent particles; cloudy plus flocculence.. 



UMBONATE, having a button-like, raised center. 



UNDULATE, border wavy, with shallow sinuses. 



VERRUCOSE, growth wart-like, with wart-like prominences. 



VERMIFORM-CONTOURED, growth like a mass of worms, or intes- 

 tinal coils. 



VILLOUS, growth beset with hair-like extensions. 



VISCID, growth follows the needle when touched and withdrawn, 

 sediment on shaking rises as a coherent swirl. 



ZOOGLOEAE, firm gelatinous masses of bacteria, one of the most 

 typical examples of which is the Streptococcus mesenterioides of sugar 

 vats (Leuconostoc mesenterioides), the bacterial chains being sur- 

 rounded by enormously thickened firm covering, inside of which 

 there may be one or many groups of the bacteria. 



NOTES. 



(1) For decimal system of group numbers see Table I. This 

 will be found useful as a quick method of showing close relationships 

 inside the genus, but is not a sufficient characterization of any organism. 



(2) The morphological characters shall be determined and described 

 from growths obtained upon at least one solid medium (nutrient agar) 

 and in at least one liquid medium (nutrient broth). Growths at 37 C 

 shall be in general not older than 24 to 48 hours, and growths at 20 C. not 

 older than 48 to 72 hours. To secure uniformity in cultures, in all cases 

 preliminary cultivation shall be practised as described in the revised 

 Report of the Committee on Standard Methods of the Laboratory Sec- 

 tion of the American Public Health Association. 1905. 



(3) The observation of cultural and bio-chemical features shall 

 cover a period of at least 15 days and frequently longer, and shall be 

 made according to the revised Standard Methods above referred to. 

 All media shall be made according to the same Standard Methods. 



(4) Gelatin stab cultures shall be held for 6 weeks to determine 

 liquefaction. 



(5) Ammonia and indol tests shall be made at end of tenth day, 

 nitrite tests at end of fifth day. 



(6) Titrate with ^ NaOH, using phenolphthalein as an indicator: 

 make titrations at same times from blank. The difference gives the 

 amount of acid produced. 



