XIV CONTENTS. 



CHAPTER III. 



PAGE 



MICROSCOPICAL EXAMINATION OF BACTERIA IN 

 LIQUIDS, IN CULTIVATIONS ON SOLID MEDIA, AND 

 IN TISSUES .45 



a. Examination in the fresh state . . . . .46 



b. Cover-glass preparations ; Methods of Ehrlich, Babes, and His . 48 



c. Cover-glass impressions . . . . . S 2 



CHAPTER IV. 

 FREPARA TION AND STAINING OF TISSUE SECTIONS . 54 



a. Methods of hardening and decalcifying preparations . . 54 



b. Methods of embedding, fixing, and cutting . . -55 



c. General principles of staining bacteria in tissue sections : methods 



of Weigert, Gram, and Weigert-Ehrlich . . -57 



CHAPTER V. 



PREPARATION OF NUTRIENT MEDIA AND METHODS 



OF CULTIVATION 62 



SOLID MEDIA : 



a. Preparation of sterile gelatine-, and agar-agar-peptone -broth . 64 



b. Methods of employing nutrient jelly in test-tube- and plate-cultiva- 



tions ........ 69 



c. Preparation and employment of sterilised potatoes, potato-paste, 



bread-paste, vegetables, fruit, and white of egg . . 82 



d. Preparation and employment of sterile blood serum . . 86 



LIQUID MEDIA : 



e. Preparation of sterilised bouillon, liquid blood serum, urine, milk, 



vegetable infusions, and artificial nourishing liquids . . 88 



/ Methods of storing and employing liquid media ; Lister's flasks, 

 Aitken's test-tubes, Sternberg's bulbs, Pasteur's apparatus, 

 Miquel's bulbs; Drop cultures; Warm stages . . 91 



