IO BACTERIOLOGY. 



5. Alum carmine (Grenacher). 



Carmine . ..... i 



Five per cent, solution of alum . 100 

 Boil twenty minutes, filter when cold. 



6. Ammonia, strong. 

 . 7. Aniline. 



8. Aniline water. 



Distilled water . . . 100 



Aniline ... 5 



Shake well and filter emulsion. 



9. Bismarck brown. 



(a) Concentrated solution in equal parts of gly- 

 cerine and water. 



(') Aqueous solution. 



Bismarck brown . . 2 



Alcohol 15 



Distilled water . 85 



10. Borax carmine (Grenacher). 



Borax ...... 2 



Carmine ..... '5 



Distilled water . . . .100 



To the dark purple solution add a 5 p. c. sol. of 

 acetic acid until a red colour is produced ; set aside 

 24 hours, filter, and add a drop of carbolic acid. 



