230 BACTERIOLOGY. 



number of rods. The bacilli were observed in 

 sections of a tumour, rhinoscleroma, which develops 

 on the lip and on the nasal and pharyngo-laryngeal 

 .regions. 



METHOD OF STAINING THE BACILLUS OF RHINO- 

 SCLEROMA. 



Method of Cornil and Alvarez : 



Sections are immersed in a solution of methyl-violet (B) 

 for twenty-four to forty-eight hours, with or without the 

 addition of aniline-water; are then decolorised after treat- 

 ment with the solution of iodine in iodide of potassium. 

 If the sections are left to decolorise in alcohol for forty- 

 eight hours the capsule is rendered visible. 



Bacterium in diphtheria of man (Bacillus 

 of diphtheria, Loffler). Rods about the same length 

 as the tubercle bacillus, but about twice as thick; 

 the longer ones consist of single individuals 

 linked together. Spores not observed. They 

 were cultivated in a mixture consisting of three 

 parts of calf's or lamb's blood serum, to which 

 was added one part of neutralised veal broth, 

 containing i per cent, peptone, i per cent, grape 

 sugar, ij per cent, common salt. Cultivated in 

 5 per cent, gelatine at 20 22 C., the rods 

 developed into irregular involution-forms. In- 

 oculation gave doubtful results. The bacillus was 

 isolated from diphtheritic membrane.* They were 

 particularly noticeable in those typical cases 



* Loffler, MittheiL a.d. K. Gesundheitsamte (Microfiarasites in 

 Disease, New Syd. Society). 



