SYSTEMATIC AND DESCRIPTIVE. 253 



thread (Figs. 92 and 93).* In about eight days, 

 however, liquefaction takes place along the whole 

 of the needle track. 



On a sloping surface of agar-agar the cultivation 

 develops as a white, semi-transparent layer, with well- 

 defined margin. In potato-cultivations the microbe 









'-" 



F IG . 89. SECTION OF THE Mucous MEMBRANE OF A CHOLERA INTESTINE, 

 X 600. A tubular gland (a) is divided transversely ; in its interior (/>) 

 and between the epithelium and the basement membrane (<:) are numerous 

 comma bacilli [after Koch], 



will only grow at the temperature of the blood 

 (37C.), forming a slightly brown, transparent layer. 

 Inoculation of a cultivation of the bacillus in the 

 duodenum of guinea-pigs, with t and without $ 

 ligation of the bile duct, has given positive results. 

 More recently these results have been confirmed 

 by the following method. Five ccm. of a 5 per 



* from Remarks on the Comma- Bacillus of Koch. Lancet. 1885. 

 t Nicati et Rietsch, Com. a P Academic de Medecine. 1884. 

 J Van Ermengem, Le Microbe du Cholera Asiatique. 1885. 



