SYSTEMATIC AND DESCRIPTIVE. 26/ 



preparations may be made.* Cover-glass preparations 

 and sections may be stained by Ehrlich's method (p. 50), 

 or the latter by the following process : 



M ethod of Babes. \ Preparations are stained in a solution 

 of rosaniline hydrochlorate in aniline-water. Decolorise 

 in 33 per cent, hydrochloric acid, and after-stain with 

 methylene blue. 



Bacillus in syphilis, Lustgarten. :); Rods re- 

 sembling the bacilli of leprosy and tuberculosis, 

 3 4 fji long, *8 n thick. Two or more colourless, 

 ovoid points in the course of the rod are visible with 

 a high power ; it is thought that they are possibly 

 spores. The bacilli are always found in the interior 

 of nucleated cells which are more than double the 

 size of leucocytes. They have been observed in the 

 discharge of the primary lesion, and in hereditary 

 affections of tertiary gummata. Some observers 

 state that an identical bacillus is found in normal 

 secretions, and others || have described a bacillus 

 associated with specific lesions, which is stated to 

 differ from the above in its behaviour towards stain- 

 ing reagents. 



METHOD OF STAINING THE BACILLUS OF SYPHILIS. 

 Method of Lustgarten : 



Sections are placed for from twelve to twenty-four hours 

 in the following solution, at the ordinary temperature of 



* Manson, Lancet. 1884. 



t Babes, Compt. Rend, de V Acad. d. Sc. 1883. 



\ Lustgarten, Die Syphilisbacillen. Mit 4 Tafeln. 1885. 



Alvarez et Tavel, " Recherches sur le Bacille de Lustgarten," 

 Archiv de Phys. Norm, et Path., 17 ; Klemperer, " Ueber Syphilis 

 und Smegma Bacillen," Deutsche Med. Woch. 1885. 



|| Eves and Lingard, Lancet, April loth, 1886. 



