2.68 .,, , . _ BACTERIOLOGY. 



the VecflHrid-fkially the solution is warmed for two hours 

 at 60 C. : 



Concentrated alcoholic solution of gentian-violet . 1 1 

 Aniline water . ...... 100 



The sections are then placed for a few minutes in 

 absolute alcohol, and from this transferred to I 5 per cent. 

 solution of permanganate of potash. After ten minutes 

 they are immersed for a moment in a pure concentrated 

 solution of sulphurous acid. If the section is not com- 

 pletely decolorised, immersion in the alcohol and in the 

 acid bath must be repeated three or four times. The 

 sections are finally dehydrated with absolute alcohol, 

 cleared with clove-oil, and mounted in Canada balsam. 



Method of De Giacomi : 



Cover-glass preparations are stained with hot solution 

 of fuchsine containing a few drops of perchloride of iron. 

 They are then decolorised in strong perchloride of iron 

 and after-stained with vesuvin or bismarck brown. 



Method of Doutrelepont and Schiltz : 



Sections are stained in a weak aqueous solution of 

 gentian violet and after-stained with safranin. 



Bacillus typhosus, Eberth (Bacillus in typhoid 

 fever). Rods, '2 p, broad, and forming filaments 

 up to 50 ju long ; * ort rods, short, rounded at their 

 ends, and occasionally constricted in the middle ; 

 some exhibiting spore-formation. These bacilli 

 have been observed in inflamed Peyer's glands, in 

 the spleen, mesenteric glands, and the lungs in 

 fatal cases of typhoid fever. More recently J a 

 bacillus has been cultivated on several plates of 



* Kleb's Arch, /. Experimental Pathol. 1880. 



t Eberth, Virchoivs Archiv, Bd. 83. 



\ Gaffky, Mittheil. a. d. K. Gesundheitsamte. 1884. 



